How TOPS 40567-80-4, a chromogenic substrate, becomes the "golden eye" for creatinine detection

The kidneys are important excretory organs in the human body, and their functional status directly affects overall health. With the accelerated pace of life and the rising incidence rate of chronic diseases, the number of people with abnormal renal function continues to expand. Early diagnosis and regular monitoring are particularly important. Among numerous indicators for evaluating renal function, creatinine testing has become the gold standard for assessing glomerular filtration function due to its stability, sensitivity, and clinical applicability. To achieve accurate and efficient creatinine detection, the outstanding contribution of the key substance, the chromogenic substrate N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt (TOPS reagent), is indispensable.

The chemical reaction nature of creatinine detection

The main reaction principle of creatinine detection is that creatinine reacts with alkaline picric acid to produce orange red compounds, which are quantitatively analyzed by colorimetric method. However, traditional methods have limitations in low specificity and are susceptible to interference from other substances in the blood. Modern enzymatic detection uses an enzyme system composed of creatine kinase, creatine kinase, and sarcosine oxidase to convert creatinine into hydrogen peroxide, which is then measured through a colorimetric reaction. The core of this process is the outstanding performance of TOPS as a chromogenic substrate.

TOPS Powder

Chemical characteristics and advantages of TOPS

TOPS, as a novel Trinder's reagent, has the characteristics of good water solubility, high stability, and excellent color sensitivity. In the presence of peroxidase, TOPS reacts with hydrogen peroxide to produce a red quinone dye, with a maximum absorption peak around 550nm and a high molar absorptivity of 3.74 × 10 ^ 4. The color reaction is rapid and stable. Compared with other chromogenic substrates, TOPS has higher detection sensitivity and lower detection limit, which can accurately reflect trace changes in creatinine in samples and provide more reliable detection results for clinical use.

The application advantages of TOPS

1. High detection accuracy

The high sensitivity of TOPS enables it to detect lower concentrations of creatinine changes, which is crucial for the detection of early renal dysfunction. Research has shown that the creatinine detection method using TOPS has a correlation coefficient of over 0.99 compared to traditional methods, and has excellent consistency with the reference method of isotope dilution mass spectrometry (ID-MS), greatly improving the accuracy of estimating glomerular filtration rate.

2. Strong anti-interference ability

The composition of human blood is complex and contains various substances that may interfere with the detection results. The enzymatic detection based on TOPS has strong specificity and is not easily affected by common interfering substances such as bilirubin, vitamin C, and protein. The false positive and false negative rates are significantly reduced, ensuring the reliability of the detection results.

3. Easy to operate process

TOPS color reaction is fast and stable, with short incubation time. After color development, it can remain stable for a long time, which is beneficial for the automation of processing and detection of large quantities of samples. This feature significantly improves laboratory work efficiency, shortens report issuance time, and provides diagnostic evidence for clinical doctors and patients more quickly.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as a high-quality developer and manufacturer of chromogenic substrates, can provide high-quality diagnostic reagent raw materials for in vitro diagnostic kit manufacturers. Desheng has professional experimental equipment and technical personnel, and can provide good customized and after-sales services. If you are also interested in TOPS and other new trinder's reagents, please click on the official website to learn more details or contact me!