The key role of biological buffer DIPSO 68399-80-4 in chromatographic separation

In the precise world of chromatographic analysis, separation efficiency and accuracy of results are the core goals that researchers tirelessly pursue. In this sorting competition of the microscopic world, the biological buffer 3- [N, N-di (hydroxyethyl) amino] -2-hydroxypropanesulfonic acid (DIPSO buffer) is playing a key role in optimizing separation conditions and ensuring experimental stability due to its unique chemical properties. This article will analyze the core value of DIPSO in chromatographic separation from three dimensions: pH stability regulation, improvement of anti-interference ability, and compatibility advantages.

1, Accurate pH regulation

The essence of chromatographic separation is to utilize the distribution difference between the stationary phase and the mobile phase to achieve separation, and pH value, as the core parameter affecting the distribution coefficient, directly determines the charged state, solubility, and interaction with the stationary phase of the target substance. The molecular structure of DIPSO contains both amino (basic group) and sulfonic acid (acidic group) groups. This zwitterionic property endows it with a wide range of buffering ability (pH 6.4-7.8), which can effectively resist external acid-base interference and maintain the dynamic balance of the system pH during mobile phase preparation or gradient elution.

DIPSO Powder

For example, in ion exchange chromatography, the separation of biomolecules such as proteins highly relies on the electrostatic interaction between their surface charges and the stationary phase. If the pH fluctuates beyond the protein's isoelectric point (pI) range, it may cause a change in its charge state, leading to peak broadening, tailing, or even inability to wash off. The addition of DIPSO can stabilize pH and ensure that the target substance is always in the optimal charge state, significantly improving the repeatability and resolution of separation.

2, Anti-interference barrier

Actual samples (such as biological fluids and environmental samples) often contain interfering substances such as metal ions and organic impurities, which may disrupt the stability of chromatographic separation by competing for binding sites with the target substance and changing the ion strength of the mobile phase. In the molecular structure of DIPSO, the hydroxyethyl amino group can form a hydrogen bond network, while the sulfonic acid group inhibits metal ion adsorption through electrostatic repulsion, thereby constructing a dual anti-interference barrier.

In reverse phase chromatography, the addition of DIPSO can reduce the non-specific binding of metal ions in the sample to the silica gel matrix stationary phase, avoiding a decrease in column efficiency; In hydrophilic interaction chromatography (HILIC), the interaction between hydroxyl groups and polar stationary phases can optimize the hydration layer structure, enhance the retention and separation of polar compounds. This "purification" effect makes DIPSO particularly suitable for direct analysis of complex matrix samples, without the need for tedious preprocessing steps.

3, Compatibility advantage

Chromatographic separation often involves various additives such as organic solvents (such as acetonitrile, methanol), ion pair reagents, surfactants, etc. The differences in chemical properties of these components may cause problems such as buffer precipitation and peak shape distortion. DIPSO exhibits strong compatibility due to its excellent solubility and chemical stability.

Product packaging

As a supplier of biological buffering agents such as DIPSO, Hubei Xindesheng ensures that every batch of DIPSO buffering agents has high purity and stable performance. New Desheng can meet the requirements in various fields, ensuring timely and accurate delivery of products to customers with sufficient inventory and fast logistics distribution. If you are interested, please feel free to click on the website for consultation at any time!