Weak binding characteristics and effects of biological buffer Bicine on metal ions

For a long time, some studies have assumed that Bicine cannot interact with metal ions. However, an increasing number of facts indicate that Bicine can form stable binary or ternary complexes with metal ions, exhibiting certain chelating properties. The molecular structure of Bicine buffer is similar to that of glycine. This structure reduces the hydrolysis ability of the amino end, enhances overall stability, and also creates conditions for its binding with metal ions.

Research has shown that Bicine can chelate with heavy metal ions such as copper (Cu), cadmium (Cd), and lead (Pb). However, this chelation effect is relatively weak and does not significantly interfere with the normal function of metal ions in most biochemical reactions, especially enzymatic reactions. In enzymatic reactions, metal ions often act as cofactors or coenzymes in the catalytic process, and their activity is highly influenced by various factors, with pH being one of the key factors. Extreme pH values can alter the binding state between metal ions and enzymes, thereby affecting catalytic activity. Bicine maintains pH stability in the solution while having weak chelation effect on metal ions, making it an ideal choice for enzymatic reactions that require the retention of metal ion activity. For example, in the serum guanine enzyme assay, the presence of Bicine can provide a stable pH environment for enzymatic reactions, and due to its weak binding properties with metal ions, it will not interfere with the role of metal ions in the reaction, ensuring the accuracy of the measurement results.

From an application perspective, this characteristic of Bicine enables it to be widely used in many fields. In protein crystallization solution, Bicine can maintain the pH stability of the system without affecting the necessary interactions between proteins and metal ions due to excessive chelation of metal ions, which is beneficial for the growth and stability of protein crystals. Bicine acts as a buffer when extracting enzymes from various plant species, ensuring a suitable pH environment and maintaining metal ions in a free state that can participate in the composition of enzyme active centers or promote enzyme activity. In polymerase chain reaction (PCR), Bicine buffer system provides stable acid-base conditions for the reaction, while not hindering the reaction due to strong chelation of metal ions (such as magnesium ions, activators of Taq DNA polymerase in PCR reaction).

Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields.

The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details!

Overall, the weak binding properties of the biological buffer Bicine with metal ions have more advantages than disadvantages in most biochemical and molecular biology applications. It provides a stable and suitable environment for numerous biochemical reactions that require the participation of metal ions, ensuring the authenticity and reliability of experimental results. With the continuous deepening of research and the expansion of application fields, fully understanding and reasonably utilizing the characteristics of Bicine and metal ions will inject new vitality into the development of related fields.

The Bicine buffer produced by Hubei Xindesheng Material Technology Co., Ltd. has a low chloride ion content and all indicators meet relevant standards. In addition to Bicine buffer, Desheng actively researches and develops dozens of biological buffers such as TRIS and Hepes commonly used in the market. If you are interested, please click on the Desheng official website to learn more details!